Anticorps Recombinant de lapin anti-CD42b

CD42b Recombinant Antibody for FC

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

FC

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

AK2

N° de cat : CL594-65588

Synonymes

GP1BA, GP1B, CD42b alpha, BSS, Antigen CD42b-alpha

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Galerie de données de validation

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  FC experiment of human peripheral blood platelets using CL594-65588

  1x10^6 human peripheral blood platelets were surface stained with 5 ul CoraLite®594 Anti-Human CD42b (AK2) Mouse IgG2a Recombinant Antibody (CL594-65588, Clone: AK2) (red), or CoraLite®594 Mouse IgG2a Isotype Control (C1.18.4) (CL594-65208, Clone: C1.18.4) (blue). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: plaquettes de sang périphérique humain,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-65588 cible CD42b dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Plaquettes humaines
• Nom complet: glycoprotein Ib (platelet), alpha polypeptide
• Masse moléculaire calculée: 626 aa, 69 kDa
• Numéro d’acquisition GenBank: BC027955
• Symbole du gène: CD42b
• Identification du gène (NCBI): 2811
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD42b, also known as platelet glycoprotein Ib alpha chain (GPIb alpha), is a type I transmembrane glycoprotein of 135-145 kDa (PMID: 2656709). It is expressed on platelets and megakaryocytes. CD42b and CD42c (GPIb beta) are linked by a disulphide bond to form GPIb (PMID: 7660135). GPIb forms a noncovalent complex with CD42a (GPIX) and CD42d (GPV) and acts as a receptor for von Willebrand factor (vWF) and thrombin (PMID: 7660135; 3759960). The GPIb-V-IX complex mediates vWF-dependent platelet adhesion to blood vessels. Defects in the expression of CD42b result in Bernard-Soulier syndromes and platelet-type von Willebrand disease (PMID: 9371310).

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