Anticorps Monoclonal anti-CD43

CD43 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2A11D6

N° de cat : 66224-1-PBS

Synonymes

2A11D6, CD43 cytoplasmic tail, Galactoglycoprotein, GALGP, GPL115

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Galerie de données de validation

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  WB analysis of Jurkat using 66224-1-Ig (same clone as 66224-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66224-1-Ig (CD43 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66224-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 66224-1-Ig (same clone as 66224-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 66224-1-Ig (CD43 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66224-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66224-1-Ig (same clone as 66224-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66224-1-Ig (CD43 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66224-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66224-1-Ig (same clone as 66224-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66224-1-Ig (CD43 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66224-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66224-1-Ig (same clone as 66224-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66224-1-Ig (CD43 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66224-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 66224-1-Ig (same clone as 66224-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66224-1-Ig (CD43 antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66224-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 66224-1-Ig (same clone as 66224-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66224-1-Ig (CD43 antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66224-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66224-1-Ig (same clone as 66224-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66224-1-Ig (CD43 antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66224-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66224-1-Ig (same clone as 66224-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66224-1-Ig (CD43 antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66224-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 66224-1-Ig (same clone as 66224-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD43 antibody (66224-1-Ig, Clone: 2A11D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66224-1-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 66224-1-Ig (same clone as 66224-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using 66224-1-Ig (CD43 antibody) at dilution of 1:400 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66224-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66224-1-PBS cible CD43 dans les applications de WB, IHC, IF/ICC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD43 Protéine recombinante Ag23595
• Nom complet: sialophorin
• Masse moléculaire calculée: 400 aa, 43 kDa
• Poids moléculaire observé: 115 kDa
• Numéro d’acquisition GenBank: BC012350
• Symbole du gène: CD43
• Identification du gène (NCBI): 6693
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD43, also named leukosialin, sialophorin, galactoglycoprotein, leukocyte sialoglycoprotein, SPN, and is a mucin-like type I transmembrane protein. In humans, it is expressed in haematopoietic cells, including T lymphocytes, monocytes, granulocytes, natural killer cells, platelets, and haematopoietic stem cells, with the exception of mature erythrocytes and B cell subpopulations. The unglycosylated form of CD43 migrates on SDS-PAGE with a molecular weight of 54 kDa (PMID: 2943740), whereas CD43 from different haematopoietic cell lines displays increased molecular weights since it is glycosylated with O-linked chains that differ in core structure and sialylation.

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