Anticorps Polyclonal de lapin anti-CD44

• Phare
• Validé par KD/KO

CD44 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, Neutralization, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

279

Avis (1)

N° de cat : 15675-1-AP

Synonymes

CD44, CD44 antigen, CDW44, CSPG8, ECMR III, Epican, HCELL, Heparan sulfate proteoglycan, Hermes antigen, HUTCH I, Hyaluronate receptor, IN, LHR, MC56, MDU2, MDU3, MIC4, MUTCH I, PGP 1, PGP I, Pgp1, Phagocytic glycoprotein 1, Phagocytic glycoprotein I

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Galerie de données de validation

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  WB analysis of HeLa cells using 15675-1-AP

  WB result of CD44 antibody (15675-1-AP, 1:6000) with si-Control and si-CD44 transfected HeLa cells.

• 

  WB analysis using 15675-1-AP

  MDA-MB-231 (CD44+), HeLa (CD44+), A549 (CD44+), MCF-7, Jurkat cell lysates were subjected to SDS PAGE followed by western blot with 15675-1-AP (CD44 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15675-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15675-1-AP (CD44 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 15675-1-AP

  IP Result of anti-CD44 (IP:15675-1-AP, 4ug; Detection:15675-1-AP 1:2000) with HeLa cells lysate 2400ug.

• 

  IHC staining of human lung cancer using 15675-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15675-1-AP (CD44 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 15675-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15675-1-AP (CD44 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15675-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15675-1-AP (CD44 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 15675-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 15675-1-AP (CD44 antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 15675-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 15675-1-AP (CD44 antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15675-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15675-1-AP (CD44 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 15675-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 15675-1-AP (CD44 antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of UCMSCs using 15675-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed UCMSCs cells using CD44 antibody (15675-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A375 using 15675-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A375 cells using CD44 antibody (15675-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HUVEC using 15675-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using CD44 antibody (15675-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 15675-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 15675-1-AP (CD44 antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 15675-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 15675-1-AP (CD44 antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human colon cancer using 15675-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using 15675-1-AP (CD44 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 15675-1-AP

  1X10^6 HeLa cells were surface stained with 0.4 ug Anti-Human CD44 (15675-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were not fixed.

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Applications testées

• Résultats positifs en WB: cellules MDA-MB-231, cellules A549, cellules HeLa, cellules HUVEC
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer de l'œsophage humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa, cellules A375, cellules HUVEC, tissu de cancer du côlon humain
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 14 publications below
• WB: See 187 publications below
• IHC: See 75 publications below
• IF: See 71 publications below
• IP: See 1 publications below
• FC: See 9 publications below
• CoIP: See 2 publications below

Informations sur le produit

15675-1-AP cible CD44 dans les applications de WB, Neutralization, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: bovin, Humain, cerf Sika
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD44 Protéine recombinante Ag8143
• Nom complet: CD44 molecule (Indian blood group)
• Masse moléculaire calculée: 742 aa, 82 kDa
• Poids moléculaire observé: 80-95 kDa
• Numéro d’acquisition GenBank: BC004372
• Symbole du gène: CD44
• Identification du gène (NCBI): 960
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands. Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. This protein exists in multiple forms (with molecular weight ranging from 16 kDa to over 200 kDa) generated by alternative RNA splicing and extensive post-translational modifications.

Publications

Species	Application	Title
	IF	Nature; ADAR1 masks the cancer immunotherapeutic promise of ZBP1-driven necroptosis.; Authors - Ting Zhang
mouse	IF	Nat Commun; Hyperthermia-triggered biomimetic bubble nanomachines; Authors - Junbin Gao
mouse	IF	Adv Sci (Weinh); The Critical Role of The Piezo1/β-catenin/ATF4 Axis on The Stemness of Gli1+ BMSCs During Simulated Microgravity-Induced Bone Loss; Authors - Yuxiang Hu
human	WB,IHC,IF	Int J Oral Sci; LIMP-2 enhances cancer stem-like cell properties by promoting autophagy-induced GSK3β degradation in head and neck squamous cell carcinoma; Authors - Yuantong Liu
mouse	WB	Acta Pharm Sin B; Engineering cannabidiol synergistic carbon monoxide nanocomplexes to enhance cancer therapy via excessive autophagy; Authors - Chang Xiao
human	WB	Biomaterials; Hyaluronan decorated layer-by-layer assembled lipid nanoparticles for miR-181a delivery in glioblastoma treatment; Authors - Victor Passos Gibson

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kishor (Verified Customer) (06-23-2019)

This antibody works very good at 1:1000 in WB over night at 4o C and 1:200 for IF in cultured cells.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:1000 and 1:200
• Cell Tissue Type: Human cell lines

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