Anticorps Polyclonal de lapin anti-CD44

CD44 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 30854-1-AP

Synonymes

CD44 antigen, CDw44, CSPG8, ECMR III, ECMR-III

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Galerie de données de validation

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  WB analysis using 30854-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30854-1-AP (Cd44 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 30854-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 30854-1-AP (CD44 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 30854-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 30854-1-AP (CD44 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 30854-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 30854-1-AP (CD44 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 30854-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 30854-1-AP (CD44 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of RAW 264.7 using 30854-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using CD44 antibody (30854-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: J774A.1 cells, cellules RAW 264.7
• Résultats positifs en IHC: tissu splénique de souris, tissu de côlon de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules RAW 264.7,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

30854-1-AP cible CD44 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD44 Protéine recombinante Ag33515
• Nom complet: CD44 antigen
• Masse moléculaire calculée: 86kd
• Poids moléculaire observé: 90 kDa
• Numéro d’acquisition GenBank: NM_009851
• Symbole du gène: Cd44
• Identification du gène (NCBI): 12505
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.

Publications

Species	Application	Title
rat	IHC	Inflamm Regen; Macrophage depletion in inflamed rat knees prevents the activation of synovial mesenchymal stem cells by weakening Nampt and Spp1 signaling; Authors - Hayato Kodama
rat	WB	Pharmacol Res; Paclitaxel-Ang-2-functionalized bionic mesoporous selenium nanoparticles for targeted therapy of glioma; Authors - Huan Shi
mouse	IF	Adv Sci (Weinh); Neutrophil-Mimicking Nanomedicine Eliminates Tumor Intracellular Bacteria and Enhances Chemotherapy on Liver Metastasis of Colorectal Cancer; Authors - Yanan Niu

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