Anticorps Recombinant de lapin anti-CD44

CD44 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241648D1

N° de cat : 84369-5-PBS

Synonymes

Hermes antigen, GP90 lymphocyte homing/adhesion receptor, Extracellular matrix receptor III, ECMR-III, CD44 antigen

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Galerie de données de validation

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  WB analysis of rat spleen using 84369-5-RR (same clone as 84369-5-PBS)

  rat spleen tissue was subjected to SDS PAGE followed by western blot with 84369-5-RR (CD44 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84369-5-PBS in a different storage buffer formulation.

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  IHC staining of rat spleen using 84369-5-RR (same clone as 84369-5-PBS)

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 84369-5-RR (CD44 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84369-5-PBS in a different storage buffer formulation.

• 

  IHC staining of rat spleen using 84369-5-RR (same clone as 84369-5-PBS)

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 84369-5-RR (CD44 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84369-5-PBS in a different storage buffer formulation.

• 

  IF Staining of NR8383 using 84369-5-RR (same clone as 84369-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NR8383 cells using CD44 antibody (84369-5-RR, Clone: 241648D1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84369-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84369-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84369-5-RR against Rat CD44 were performed. The affinity constant is 0.332 nM.

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Informations sur le produit

84369-5-PBS cible CD44 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons rat

• Réactivité: rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: Cd44 molecule
• Masse moléculaire calculée: 40 kDa
• Poids moléculaire observé: 80 kDa
• Numéro d’acquisition GenBank: XM_006234631.3
• Symbole du gène: Cd44
• Identification du gène (NCBI): 25406
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.

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