Anticorps Recombinant de lapin anti-CD44

CD44 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242345G11

N° de cat : 84854-5-PBS

Synonymes

242345G11, CD44 antigen, CDW44, CSPG8, ECMR III

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Galerie de données de validation

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  WB analysis using 84854-5-RR (same clone as 84854-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84854-5-RR (CD44 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84854-5-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 84854-5-RR (same clone as 84854-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84854-5-RR (CD44 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84854-5-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 84854-5-RR (same clone as 84854-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84854-5-RR (CD44 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84854-5-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 84854-5-RR (same clone as 84854-5-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CD44 antibody (84854-5-RR, Clone: 242345G11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84854-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84854-5-PBS

  Biolayer interferometry (BLl) kinetic assay of 84854-5-PBS against Human CD44 was performed. The affinity constant is 2.04 nM.

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  Affinity and Kinetic Characterization of 84854-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84854-5-RR against Human CD44 were performed. The affinity constant is 1.01 nM.

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Informations sur le produit

84854-5-PBS cible CD44 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD44 Protéine recombinante Eg1057
• Nom complet: CD44 molecule (Indian blood group)
• Masse moléculaire calculée: 82 kDa
• Poids moléculaire observé: 80-95 kDa
• Numéro d’acquisition GenBank: NM_000610.3
• Symbole du gène: CD44
• Identification du gène (NCBI): 960
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.

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