Anticorps Recombinant de lapin anti-CD44

CD44 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

242345G11

N° de cat : 84854-5-RR

Synonymes

ECMR-III, ECMR III, CSPG8, CDW44, CD44 antigen

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Galerie de données de validation

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  WB analysis using 84854-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84854-5-RR (CD44 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 84854-5-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84854-5-RR (CD44 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 84854-5-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84854-5-RR (CD44 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 84854-5-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CD44 antibody (84854-5-RR, Clone: 242345G11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84854-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84854-5-RR against Human CD44 were performed. The affinity constant is 1.01 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules MDA-MB-231, cellules PC-3
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84854-5-RR cible CD44 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: CD44 molecule (Indian blood group)
• Masse moléculaire calculée: 82 kDa
• Poids moléculaire observé: 80-95 kDa
• Numéro d’acquisition GenBank: NM_000610.3
• Symbole du gène: CD44
• Identification du gène (NCBI): 960
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.

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