Anticorps Recombinant de lapin anti-CD44

CD44 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242666C11

N° de cat : 85163-5-PBS

Synonymes

242666C11, CD44 antigen, CDw44, CSPG8, ECMR III

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Galerie de données de validation

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  WB analysis using 85163-5-RR (same clone as 85163-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85163-5-RR (Cd44 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85163-5-PBS in a different storage buffer formulation.

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  WB analysis using 85163-5-RR (same clone as 85163-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85163-5-RR (Cd44 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85163-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 85163-5-RR (same clone as 85163-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85163-5-RR (CD44 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85163-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 85163-5-RR (same clone as 85163-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85163-5-RR (CD44 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85163-5-PBS in a different storage buffer formulation.

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  IF Staining of RAW 264.7 using 85163-5-RR (same clone as 85163-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using Cd44 antibody (85163-5-RR, Clone: 242666C11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85163-5-PBS in a different storage buffer formulation.

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Informations sur le produit

85163-5-PBS cible CD44 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD44 Protéine recombinante Eg3159
• Nom complet: CD44 antigen
• Masse moléculaire calculée: 86 kDa
• Poids moléculaire observé: 85 kDa
• Numéro d’acquisition GenBank: NM_009851.2
• Symbole du gène: Cd44
• Identification du gène (NCBI): 12505
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.

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