Anticorps Recombinant de lapin anti-CD44

CD44 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

242345G9

N° de cat : 98333-3-RR

Synonymes

242345G9, CD44 antigen, CDW44, CSPG8, ECMR III

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Galerie de données de validation

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  FC experiment of human PBMCs using 98333-3-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD44 Rabbit RecAb (98333-3-RR, Clone:242345G9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9). Cells were then stained with APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using 98333-3-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD44 Rabbit RecAb (98333-3-RR, Clone:242345G9) (red) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98333-3-RR

  Biolayer interferometry (BLl) kinetic assays of 98333-3-RR against Human CD44 were performed. The affinity constant is 7.25 nM.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98333-3-RR cible CD44 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD44 Protéine recombinante Eg1057
• Nom complet: CD44 molecule (Indian blood group)
• Masse moléculaire calculée: 82 kDa
• Numéro d’acquisition GenBank: NM_000610.3
• Symbole du gène: CD44
• Identification du gène (NCBI): 960
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.

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