Anticorps Recombinant de lapin anti-CD44

CD44 Recombinant Antibody for FC

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

FC

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

F10-44-2

N° de cat : CL594-65608

Synonymes

CD44 antigen, CDW44, CSPG8, ECMR III, ECMR-III

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Galerie de données de validation

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  FC experiment of human PBMCs using CL594-65608

  1x10^6 human PBMCs were surface stained with CoraLite® Plus 647 Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (CL647-65569, Clone: OKT3), and 5 ul CoraLite®594 Anti-Human CD44 (F10-44-2) Mouse IgG2a RecAb (CL594-65608, Clone:F10-44-2) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4). Cells were not fixed.

• 

  FC experiment of human PBMCs using CL594-65608

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite®594 Anti-Human CD44 (F10-44-2) Mouse IgG2a RecAb (CL594-65608, Clone: F10-44-2) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-65608 cible CD44 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: s/o
• Nom complet: CD44 molecule (Indian blood group)
• Masse moléculaire calculée: 742 aa, 82 kDa
• Numéro d’acquisition GenBank: BC004372
• Symbole du gène: CD44
• Identification du gène (NCBI): 960
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.

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