Anticorps Recombinant de lapin anti-CD45.2

CD45.2 Recombinant Antibody for WB, IHC, IF-P, IF-Fro, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, IHC, IF-P, IF-Fro, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241543F9

N° de cat : 84637-1-PBS

Synonymes

Cd45, Ptprc, 241543F9, EC:3.1.3.48, LCA

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Galerie de données de validation

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  WB analysis using 84637-1-RR (same clone as 84637-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84637-1-RR (CD45.2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84637-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 84637-1-RR (same clone as 84637-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84637-1-RR (CD45.2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84637-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 84637-1-RR (same clone as 84637-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84637-1-RR (CD45.2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84637-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse spleen using 84637-1-RR (same clone as 84637-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD45.2 antibody (84637-1-RR, Clone: 241543F9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84637-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse spleen using 84637-1-RR (same clone as 84637-1-PBS)

  Immunofluorescent analysis of un-fixed frozen OCT-embedded mouse spleen tissue using CD45.2 antibody (84637-1-RR, Clone: 241543F9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84637-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84637-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84637-1-RR against Mouse CD45.2 were performed. The affinity constant is 23.9 nM.

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Informations sur le produit

84637-1-PBS cible CD45.2 dans les applications de WB, IHC, IF-P, IF-Fro, Indirect ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD45.2 Protéine recombinante Eg2467
• Nom complet: protein tyrosine phosphatase, receptor type, C
• Poids moléculaire observé: 200-220 kDa
• Symbole du gène: CD45
• Identification du gène (NCBI): 19264
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). Allelic variants of mouse CD45, CD45.1 (Ly5.1) and CD45.2 (Ly5.1), have been established as a marker system to track haematopoietic cells following congenic mouse bone marrow transplants (PMID: 28615666). CD45.2 is the common form and is expressed by most of the established strains, while CD45.1 is found in only a few like the SJL mouse strain (PMID: 3489673).

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