Anticorps Polyclonal de lapin anti-CD45

CD45 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 31243-1-PBS

Synonymes

Ptprc, EC:3.1.3.48, LCA, L-CA, Leukocyte common antigen

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Galerie de données de validation

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  WB analysis using 31243-1-AP (same clone as 31243-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31243-1-AP (CD45/PTPRC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31243-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 31243-1-AP (same clone as 31243-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31243-1-AP (CD45 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31243-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 31243-1-AP (same clone as 31243-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31243-1-AP (CD45 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31243-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse thymus using 31243-1-AP (same clone as 31243-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 31243-1-AP (CD45 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31243-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse thymus using 31243-1-AP (same clone as 31243-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 31243-1-AP (CD45 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31243-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 31243-1-AP (same clone as 31243-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 31243-1-AP (CD45 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31243-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 31243-1-AP (same clone as 31243-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 31243-1-AP (CD45 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31243-1-PBS in a different storage buffer formulation.

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  IF Staining of RAW 264.7 using 31243-1-AP (same clone as 31243-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using CD45 antibody (31243-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 31243-1-PBS in a different storage buffer formulation.

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Informations sur le produit

31243-1-PBS cible CD45 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD45 Protéine recombinante Eg0845
• Nom complet: protein tyrosine phosphatase, receptor type, C
• Masse moléculaire calculée: 145 kDa
• Poids moléculaire observé: 180 kDa
• Numéro d’acquisition GenBank: NM_001111316.2
• Symbole du gène: CD45
• Identification du gène (NCBI): 19264
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).

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