Anticorps Monoclonal anti-CD45
CD45 Monoclonal Antibody for FC, IF, IHC, WB, ELISA
Hôte / Isotype
Mouse / IgG2a
Réactivité testée
Humain, porc, rat, souris
Applications
WB, IHC, IF, FC, ELISA
Conjugaison
Non conjugué
CloneNo.
4E9B2
N° de cat : 60287-1-Ig
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | tissu splénique de porc, cellules Raji |
| Résultats positifs en IHC | tissu d'appendicite humain, tissu d'amygdalite humain, tissu de cancer du foie humain, tissu de côlon de rat, tissu de côlon humain, tissu de thymus de rat, tissu splénique de souris il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
| Résultats positifs en IF | tissu de côlon de souris, tissu d'amygdalite humain, tissu splénique de souris |
| Résultats positifs en cytométrie | cellules Raji |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochimie (IHC) | IHC : 1:4000-1:16000 |
| Immunofluorescence (IF) | IF : 1:500-1:2000 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| WB | See 1 publications below |
| IHC | See 13 publications below |
| IF | See 17 publications below |
| FC | See 7 publications below |
Informations sur le produit
60287-1-Ig cible CD45 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris
| Réactivité | Humain, porc, rat, souris |
| Réactivité citée | rat, Humain, porc, souris |
| Hôte / Isotype | Mouse / IgG2a |
| Clonalité | Monoclonal |
| Type | Anticorps |
| Immunogène | CD45 Protéine recombinante Ag13712 |
| Nom complet | protein tyrosine phosphatase, receptor type, C |
| Masse moléculaire calculée | 1304 aa, 147 kDa |
| Poids moléculaire observé | 220 kDa |
| Numéro d’acquisition GenBank | BC014239 |
| Symbole du gène | PTPRC |
| Identification du gène (NCBI) | 5788 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par protéine A |
| Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
Protein tyrosine phosphatase receptor type C (PTPRC) is also known as CD45 antigen, which was originally called leukocyte common antigen (LCA). CD45 is an enzyme required for T-cell activation through the antigen receptor consisting of two intracellular phosphatase domains, a transmembrane domain and an extracellular domain. Upon T-cell activation, CD45 recruits and dephosphorylates SKAP1 via an interaction through the first PTPase domain, which is the only domain to possess intrinsic kinase activity. However, both domains are required for appropriate phosphatase and T-cell antigen receptor activity (PMID: 8076596).
What is the molecular weight of CD45?
The molecular weight of CD45 is a 180-240 kDa.
What are the isoforms of CD45?
Six different human isoforms of CD45 have been isolated, containing all three exons (ABC isoform), two of the three exons (AB and BC isoforms), only one exon (A isoform or B isoform), or no exons (O isoform). All of the isoforms have the same eight amino acids at their amino-terminus (PMID: 12412720).
What is the tissue specificity of CD45?
CD45 is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets (PMID: 7803253).
What are the post-translational modifications of CD45?
CD45 can be heavily N- and O-glycosylated (PMID: 1702721).
What is CD45's involvement in disease?
Defects in CD45 are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID); a severe form of SCID. It is classed by the impairment of both humoral and cell-mediated immunity, leukopenia, and the low or absent levels of antibodies (PMID: 10700239). Also, genetic variants of CD45 have a role in susceptibility to multiple sclerosis (PMID: 12864992).
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for CD45 antibody 60287-1-Ig | Download protocol |
| IHC protocol for CD45 antibody 60287-1-Ig | Download protocol |
| IF protocol for CD45 antibody 60287-1-Ig | Download protocol |
| FC protocol for CD45 antibody 60287-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Interventional hydrogel microsphere vaccine as an immune amplifier for activated antitumour immunity after ablation therapy | ||
Theranostics Blockade of phosphotyrosine pathways suggesting SH2 superbinder as a novel therapy for pulmonary fibrosis. | ||
Cell Biol Toxicol Induction of Sestrin2 by pterostilbene suppresses ethanol-triggered hepatocyte senescence by degrading CCN1 via p62-dependent selective autophagy. | ||
Neural Regen Res Transplantation of fibrin-thrombin encapsulated human induced neural stem cells promotes functional recovery of spinal cord injury rats through modulation of the microenvironment | ||
Stem Cell Reports Compensation of Disabled Organogeneses in Genetically Modified Pig Fetuses by Blastocyst Complementation. | ||
Stem Cell Res Ther Hepatic progenitor cells promote the repair of schistosomiasis liver injury by inhibiting IL-33 secretion in mice. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Kenzo (Verified Customer) (01-09-2023) | A good Hematopoietic cell marker for IF. Little background observed.
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