Anticorps Monoclonal anti-CD45

CD45 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, IF-Fro, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

4E9B2

N° de cat : 60287-1-PBS

Synonymes

4E9B2, EC:3.1.3.48, GP180, L CA, LCA

---

Galerie de données de validation

• 

  WB analysis of pig spleen using 60287-1-Ig (same clone as 60287-1-PBS)

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 60287-1-Ig (CD45 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60287-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Raji using 60287-1-Ig (same clone as 60287-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 60287-1-Ig (CD45 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60287-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human appendicitis using 60287-1-Ig (same clone as 60287-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60287-1-Ig (CD45 antibody) at dilution of 1:8000 (under 0x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60287-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human appendicitis using 60287-1-Ig (same clone as 60287-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60287-1-Ig (CD45 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60287-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 60287-1-Ig (same clone as 60287-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60287-1-Ig (CD45 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60287-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 60287-1-Ig (same clone as 60287-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60287-1-Ig (CD45 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60287-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat thymus using 60287-1-Ig (same clone as 60287-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 60287-1-Ig (CD45 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60287-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat thymus using 60287-1-Ig (same clone as 60287-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 60287-1-Ig (CD45 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60287-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 60287-1-Ig (same clone as 60287-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 60287-1-Ig (CD45 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60287-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 60287-1-Ig (same clone as 60287-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 60287-1-Ig (CD45 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60287-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 60287-1-Ig (same clone as 60287-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human diffuse small B-cell lymphoma tissue slide using 60287-1-Ig (CD45 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60287-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 60287-1-Ig (same clone as 60287-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human diffuse small B-cell lymphoma tissue slide using 60287-1-Ig (CD45 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60287-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat colon using 60287-1-Ig (same clone as 60287-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 60287-1-Ig (CD45 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60287-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat colon using 60287-1-Ig (same clone as 60287-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 60287-1-Ig (CD45 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60287-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 60287-1-Ig (same clone as 60287-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60287-1-Ig (CD45 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60287-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 60287-1-Ig (same clone as 60287-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60287-1-Ig (CD45 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60287-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60287-1-Ig (same clone as 60287-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60287-1-Ig (CD45 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60287-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60287-1-Ig (same clone as 60287-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60287-1-Ig (CD45 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60287-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse colon using 60287-1-Ig (same clone as 60287-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using CD45 antibody (60287-1-Ig, Clone: 4E9B2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60287-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse colon using 60287-1-Ig (same clone as 60287-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using CD45 antibody (60287-1-Ig, Clone: 4E9B2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60287-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 60287-1-Ig (same clone as 60287-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 60287-1-Ig (CD45 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60287-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 60287-1-Ig (same clone as 60287-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 60287-1-Ig (CD45 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60287-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse spleen using 60287-1-Ig (same clone as 60287-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CD45 antibody (60287-1-Ig, Clone: 4E9B2 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60287-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse spleen using 60287-1-Ig (same clone as 60287-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CD45 antibody (60287-1-Ig, Clone: 4E9B2 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60287-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse colon using 60287-1-Ig (same clone as 60287-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse colon tissue using 60287-1-Ig (CD45 antibody) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60287-1-PBS in a different storage buffer formulation.

• 

  IF Staining of Jurkat using 60287-1-Ig (same clone as 60287-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD45 antibody (60287-1-Ig, Clone: 4E9B2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60287-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP50221-4

  Cytometric bead array standard curve of MP50221-4, CD45 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60287-1-PBS. Detection antibody: 60287-5-PBS. Standard:Ag13712. Range: 0.391-100 ng/mL.

---

Informations sur le produit

60287-1-PBS cible CD45 dans les applications de WB, IHC, IF/ICC, IF-P, IF-Fro, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD45 Protéine recombinante Ag13712
• Nom complet: protein tyrosine phosphatase, receptor type, C
• Masse moléculaire calculée: 1304 aa, 147 kDa
• Poids moléculaire observé: 220 kDa
• Numéro d’acquisition GenBank: BC014239
• Symbole du gène: CD45
• Identification du gène (NCBI): 5788
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Protein tyrosine phosphatase receptor type C (PTPRC) is also known as CD45 antigen, which was originally called leukocyte common antigen (LCA). CD45 is an enzyme required for T-cell activation through the antigen receptor consisting of two intracellular phosphatase domains, a transmembrane domain and an extracellular domain. Upon T-cell activation, CD45 recruits and dephosphorylates SKAP1 via an interaction through the first PTPase domain, which is the only domain to possess intrinsic kinase activity. However, both domains are required for appropriate phosphatase and T-cell antigen receptor activity (PMID: 8076596). 

What is the molecular weight of CD45?

The molecular weight of CD45 is a 180-240 kDa. 

What are the isoforms of CD45?

Six different human isoforms of CD45 have been isolated, containing all three exons (ABC isoform), two of the three exons (AB and BC isoforms), only one exon (A isoform or B isoform), or no exons (O isoform). All of the isoforms have the same eight amino acids at their amino-terminus (PMID: 12412720). 

What is the tissue specificity of CD45?

CD45 is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets (PMID: 7803253). 

What are the post-translational modifications of CD45?

CD45 can be heavily N- and O-glycosylated (PMID: 1702721). 

What is CD45's involvement in disease?

Defects in CD45 are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID); a severe form of SCID. It is classed by the impairment of both humoral and cell-mediated immunity, leukopenia, and the low or absent levels of antibodies (PMID: 10700239). Also, genetic variants of CD45 have a role in susceptibility to multiple sclerosis (PMID: 12864992).

{{ptg:RelatedPrimaryAntibodies}}