Anticorps Monoclonal anti-CD45

CD45 Monoclonal Antibody for IF/ICC, FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain

Applications

IF/ICC, FC

Conjugaison

Non conjugué

Publications(2)

CloneNo.

HI30

N° de cat : 65109-1-Ig

Synonymes

EC:3.1.3.48, GP180, HI30, L CA, LCA

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Galerie de données de validation

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  IF Staining of Jurkat using 65109-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD45 antibody (65109-1-Ig, Clone: HI30 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of human PBMCs using 65109-1-Ig

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD45 (HI30) (65109-1-Ig, Clone: HI30) or Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21), and PE-conjugated Goat Anti-Mouse IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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  FC experiment of human PBMCs using 65109-1-Ig

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD45 (HI30) (65109-1-Ig, Clone: HI30) (red) or Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue), and PE-conjugated Goat Anti-Mouse IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Applications testées

• Résultats positifs en IF/ICC: cellules Jurkat,
• Résultats positifs en cytométrie: CMSP humaines

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below
• FC: See 1 publications below

Informations sur le produit

65109-1-Ig cible CD45 dans les applications de IF/ICC, FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Human peripheral blood leucocytes
• Nom complet: protein tyrosine phosphatase, receptor type, C
• Numéro d’acquisition GenBank: BC014239
• Symbole du gène: CD45
• Identification du gène (NCBI): 5788
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).

Publications

Species	Application	Title
human	FC	Front Cell Dev Biol; Nrf2 Activation Is Involved in Cyclic Mechanical Stress-Stimulated Osteogenic Differentiation in Periodontal Ligament Stem Cells via PI3K/Akt Signaling and HO1-SOD2 Interaction.; Authors - Xun Xi
human	IF	Cell Discov; Integration of 3D bioprinting and multi-algorithm machine learning identified glioma susceptibilities and microenvironment characteristics; Authors - Min Tang

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