Anticorps Recombinant de lapin anti-CD45

CD45 Recombinant Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA

Conjugaison

Non conjugué

CloneNo.

6O19

N° de cat : 80297-1-PBS

Synonymes

6O19, EC:3.1.3.48, GP180, L CA, LCA

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Galerie de données de validation

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  WB analysis using 80297-1-RR (same clone as 80297-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80297-1-RR (CD45 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80297-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 80297-1-RR (same clone as 80297-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 80297-1-RR (CD45 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80297-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 80297-1-RR (same clone as 80297-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 80297-1-RR (CD45 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80297-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 80297-1-RR (same clone as 80297-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 80297-1-RR (CD45 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80297-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 80297-1-RR (same clone as 80297-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 80297-1-RR (CD45 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80297-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 80297-1-RR (same clone as 80297-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 80297-1-RR (CD45 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80297-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse spleen using 80297-1-RR (same clone as 80297-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CD45 antibody (80297-1-RR, Clone: 6O19 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80297-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse spleen using 80297-1-RR (same clone as 80297-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CD45 antibody (80297-1-RR, Clone: 6O19 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80297-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse colon using 80297-1-RR (same clone as 80297-1-PBS)

  Immunofluorescence of mouse colon: Frozen OCT-embedded mouse colon sections were stained with anti-E-cadherin antibody (20874-1-AP) labeled with CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2, green) in the frist step,anti-CD45 antibody (80297-1-RR) labeled with FlexAble CoraLite Plus 555 Kit (KFA002, orange) in the second step,anti-Collagen Type III antibody (22734-1-AP) labeled with FlexAble CoraLite647 Kit (KFA003, magenta) in the third step, and DAPI (blue). This data was developed using the same antibody clone with 80297-1-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 80297-1-RR (same clone as 80297-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD45 antibody (80297-1-RR, Clone: 6O19 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80297-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 80297-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80297-1-RR against Human CD45 were performed. The affinity constant is 1.61 nM.

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Informations sur le produit

80297-1-PBS cible CD45 dans les applications de WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD45 Protéine recombinante Ag13712
• Nom complet: protein tyrosine phosphatase, receptor type, C
• Masse moléculaire calculée: 1304 aa, 147 kDa
• Poids moléculaire observé: 220-240 kDa
• Numéro d’acquisition GenBank: BC014239
• Symbole du gène: CD45
• Identification du gène (NCBI): 5788
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD45, also known as leukocyte common antigen (LCA), B220, and T200, is a 180-240 kD single chain type I membrane glycoprotein. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 is one of the best negative selection markers for characterizing and/or isolating human mesenchymal stem cells from bone marrow and other sources. Along with the positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification.

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