Anticorps Monoclonal anti-CD45

CD45 Monoclonal Antibody for IF/ICC, FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain

Applications

IF/ICC, FC

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

Publications(1)

CloneNo.

2D1

N° de cat : CL488-65082

Synonymes

2D1, EC:3.1.3.48, GP180, L CA, LCA

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Galerie de données de validation

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  IF Staining of human peripheral blood mononuclear cells using CL488-65082

  Immunofluorescent analysis of human peripheral blood mononuclear cells (PBMCs) using CoraLite® Plus 488-conjugated Anti-Human CD45 (CL488-65082, Clone: 2D1) at dilution of 1:400.

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  FC experiment of human PBMCs using CL488-65082

  1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD45 (CL488-65082, Clone:2D1) (red), or 5 ul Control Antibody. Cells were not fixed .

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Applications testées

• Résultats positifs en IF/ICC: cellules mononucléées de sang périphérique humain,
• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

CL488-65082 cible CD45 dans les applications de IF/ICC, FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CMSP humaines
• Nom complet: protein tyrosine phosphatase, receptor type, C
• Numéro d’acquisition GenBank: BC014239
• Symbole du gène: CD45
• Identification du gène (NCBI): 5788
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).

Publications

Species	Application	Title
human	IF	Anal Chim Acta; Layer-by-layer fabrication of alginate/polyethyleneimine multilayer on magnetic interface with enhanced efficiency in immuno-capturing circulating tumor cells; Authors - Yang Xiao

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