Anticorps Recombinant de lapin anti-CD45

CD45 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

240356G10

N° de cat : 83396-5-PBS

Synonymes

CD45/PTPRC, Ptprc, 240356G10, EC:3.1.3.48, LCA

---

Galerie de données de validation

• 

  WB analysis using 83396-5-RR (same clone as 83396-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83396-5-RR (CD45 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83396-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 83396-5-RR (same clone as 83396-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83396-5-RR (CD45 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83396-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 83396-5-RR (same clone as 83396-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83396-5-RR (CD45 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83396-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 83396-5-RR (same clone as 83396-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83396-5-RR (CD45 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83396-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 83396-5-RR (same clone as 83396-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83396-5-RR (CD45 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83396-5-PBS in a different storage buffer formulation.

• 

  IF Staining of RAW 264.7 using 83396-5-RR (same clone as 83396-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CD45/PTPRC antibody (83396-5-RR, Clone: 240356G10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83396-5-PBS in a different storage buffer formulation.

• 

  IF Staining of RAW 264.7 using 83396-5-RR (same clone as 83396-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CD45/PTPRC antibody (83396-5-RR, Clone: 240356G10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83396-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83396-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83396-5-RR against Mouse CD45 were performed. The affinity constant is 0.415 nM.

---

Informations sur le produit

83396-5-PBS cible CD45 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD45 Protéine recombinante Eg0845
• Nom complet: protein tyrosine phosphatase, receptor type, C
• Masse moléculaire calculée: 145 kDa
• Poids moléculaire observé: 220 kDa
• Numéro d’acquisition GenBank: NM_001111316.2
• Symbole du gène: CD45
• Identification du gène (NCBI): 19264
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).

{{ptg:RelatedPrimaryAntibodies}}