Anticorps Monoclonal anti-CD45R (B220)

CD45R (B220) Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG2a, kappa

Réactivité testée

Humain, souris

Applications

FC

Conjugaison

PE Fluorescent Dye

CloneNo.

RA3-6B2

N° de cat : PE-65139

Synonymes

B220, CD45R, Cd45, Ptprc, EC:3.1.3.48

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Galerie de données de validation

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  FC experiment of C57BL/6 mouse splenocytes using PE-65139

  1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.2 ug PE Anti-Mouse CD45R (B220) (PE-65139, Clone: RA3-6B2) or 0.2 ug PE Rat IgG2a Isotype Control (2A3) (PE-65209, Clone: 2A3), and CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5) (CL647-65104, Clone: GK1.5). Cells were not fixed.

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  FC experiment of human PBMCs using PE-65139

  1X10^6 human PBMCs were surface stained with 0.5 ug PE Anti-Mouse CD45R (B220) (PE-65139, Clone:RA3-6B2) or Rat IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

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  FC experiment of human PBMCs using PE-65139

  1X10^6 human PBMCs were surface stained with 0.5 ug PE Anti-Mouse CD45R (B220) (PE-65139, Clone:RA3-6B2) or Rat IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: splénocytes de souris, CMSP humaines

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

PE-65139 cible CD45R (B220) dans les applications de FC et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Rat / IgG2a, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cellules tumorales pré-B induites par le virus de la leucémie murine d'Abelson
• Nom complet: protein tyrosine phosphatase, receptor type, C
• Numéro d’acquisition GenBank: BC028512
• Symbole du gène: CD45
• Identification du gène (NCBI): 19264
• Conjugaison: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45R (B220) is an isoform of CD45, expressed on murine B-lineage cells (except for plasma cells) and on a small sub-population of T cells (PMID: 6975314; 15712182). It is commonly used as a pan B-cell marker in mice. CD45R is also expressed on activated T cells and NK cell subsets (PMID: 7514808; 17923504). The RA3-6B2 clone reacts with human and mouse CD45R.

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