Anticorps Recombinant de lapin anti-CD45RA

CD45RA Recombinant Antibody for FC

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

rat

Applications

FC

Conjugaison

Non conjugué

CloneNo.

OX-33

N° de cat : 65578-1-MR

Synonymes

CD45, Lca, Ptprc, L CA, OX-33

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Galerie de données de validation

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  FC experiment of rat splenocytes using 65578-1-MR

  1x10^6 rat splenocytes were surface stained with 0.25 ug Anti-Rat CD45RA Mouse IgG2a Recombinant Antibody (65578-1-MR, Clone: OX-33) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) and FITC anti-Mouse IgG2a Antibody. Cells were then stained with APC Anti-Rat CD4. Cells were not fixed.

• 

  FC experiment of rat splenocytes using 65578-1-MR

  1x10^6 rat splenocytes were surface stained with 0.25 ug Anti-Rat CD45RA Mouse IgG2a Recombinant Antibody (65578-1-MR, Clone:OX-33) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and FITC anti-Mouse IgG2a Antibody. Cells were not fixed.

• 

  FC experiment of rat splenocytes using 65578-1-MR

  1x10^6 rat splenocytes were surface stained with 0.25 ug Anti-Rat CD45RA Mouse IgG2a Recombinant Antibody (65578-1-MR, Clone: OX-33) and FITC anti-Mouse IgG2a Antibody (left), or 0.25 ug Anti-Rat CD45RA (65234-1-Ig, Clone: OX-33) and FITC anti-Mouse IgG1 Antibody (right). Cells were then stained with APC Anti-Rat CD4. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: splénocytes de rat

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

65578-1-MR cible CD45RA dans les applications de FC et montre une réactivité avec des échantillons rat

• Réactivité: rat
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Leukocyte common antigen purified from rat splenocytes
• Nom complet: protein tyrosine phosphatase, receptor type, C
• Masse moléculaire calculée: 143 kDa
• Numéro d’acquisition GenBank: XM_006249910
• Symbole du gène: CD45
• Identification du gène (NCBI): 24699
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signaling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231).

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