Anticorps Recombinant de lapin anti-CD45RA

CD45RA Recombinant Antibody for FC

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

rat

Applications

FC

Conjugaison

Non conjugué

CloneNo.

OX-33

N° de cat : 65578-1-PBS

Synonymes

CD45, Lca, Ptprc, L CA, OX-33

---

Galerie de données de validation

• 

  FC experiment of rat splenocytes using 65578-1-MR (same clone as 65578-1-PBS)

  1x10^6 rat splenocytes were surface stained with 0.25 ug Anti-Rat CD45RA Mouse IgG2a Recombinant Antibody (65578-1-MR, Clone: OX-33) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) and FITC anti-Mouse IgG2a Antibody. Cells were then stained with APC Anti-Rat CD4. Cells were not fixed. This data was developed using the same antibody clone with 65578-1-PBS in a different storage buffer formulation.

• 

  FC experiment of rat splenocytes using 65578-1-MR (same clone as 65578-1-PBS)

  1x10^6 rat splenocytes were surface stained with 0.25 ug Anti-Rat CD45RA Mouse IgG2a Recombinant Antibody (65578-1-MR, Clone:OX-33) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and FITC anti-Mouse IgG2a Antibody. Cells were not fixed. This data was developed using the same antibody clone with 65578-1-PBS in a different storage buffer formulation.

• 

  FC experiment of rat splenocytes using 65578-1-MR (same clone as 65578-1-PBS)

  1x10^6 rat splenocytes were surface stained with 0.25 ug Anti-Rat CD45RA Mouse IgG2a Recombinant Antibody (65578-1-MR, Clone: OX-33) and FITC anti-Mouse IgG2a Antibody (left), or 0.25 ug Anti-Rat CD45RA (65234-1-Ig, Clone: OX-33) and FITC anti-Mouse IgG1 Antibody (right). Cells were then stained with APC Anti-Rat CD4. Cells were not fixed. This data was developed using the same antibody clone with 65578-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

65578-1-PBS cible CD45RA dans les applications de FC et montre une réactivité avec des échantillons rat

• Réactivité: rat
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Leukocyte common antigen purified from rat splenocytes
• Nom complet: protein tyrosine phosphatase, receptor type, C
• Masse moléculaire calculée: 143 kDa
• Numéro d’acquisition GenBank: XM_006249910
• Symbole du gène: CD45
• Identification du gène (NCBI): 24699
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C.

Informations générales

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signaling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231).

{{ptg:RelatedPrimaryAntibodies}}