Anticorps Monoclonal anti-CD45RA

CD45RA Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain

Applications

FC

Conjugaison

Atlantic Blue™ Fluorescent Dye

CloneNo.

F8-11-13

N° de cat : AB-65226

Synonymes

B220, CD45, CD45R, CD45RA, GP180, L CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200



Applications testées

Résultats positifs en cytométrieCMSP humaines,

Dilution recommandée

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

AB-65226 cible CD45RA dans les applications de FC et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Mouse / IgG1, kappa
Clonalité Monoclonal
Type Anticorps
Immunogène Human T lymphocytes
Nom complet protein tyrosine phosphatase, receptor type, C
Masse moléculaire calculée 1304 aa, 147 kDa
Numéro d’acquisition GenBankBC014239
Symbole du gène PTPRC
Identification du gène (NCBI) 5788
Conjugaison Atlantic Blue™ Fluorescent Dye
Excitation/Emission maxima wavelengths404 nm / 458 nm
Forme Liquide
Méthode de purification Purification par affinité
Tampon de stockage PBS with 0.09% sodium azide and 0.5% BSA.
Conditions de stockageStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231).

Protocole

Product Specific Protocols
FC protocol for Atlantic Blue™ CD45RA antibody AB-65226Download protocol
Standard Protocols
Click here to view our Standard Protocols