Anticorps Recombinant de lapin anti-CD45RO

CD45RO Recombinant Antibody for IHC, FC

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

IHC, FC

Conjugaison

Non conjugué

CloneNo.

UCHL1

N° de cat : 65613-1-MR

Synonymes

CD45, EC:3.1.3.48, GP180, L CA, LCA

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Galerie de données de validation

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  IHC staining of human tonsillitis using 65613-1-MR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 65613-1-MR (CD45RO antibody) at dilution of 1:1200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 65613-1-MR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 65613-1-MR (CD45RO antibody) at dilution of 1:1200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of human PBMCs using 65613-1-MR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD45RO (UCHL1) Mouse IgG2a RecAb (65613-1-MR, Clone:UCHL1) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4), and PE-conjugated Goat Anti-Mouse IgG.Cells were co-stained with CoraLite® Plus 647 Anti-Human CD45RA (HI100) (CL647-65108, Clone: HI100).Cells were not fixed.

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  FC experiment of human PBMCs using 65613-1-MR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD45RO (UCHL1) Mouse IgG2a RecAb (65613-1-MR, Clone: UCHL1) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue), and PE-conjugated Goat Anti-Mouse IgG. Cells were not fixed.

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Applications testées

• Résultats positifs en IHC: tissu d'amygdalite humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:600-1:2400

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

65613-1-MR cible CD45RO dans les applications de IHC, FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Lignée de lymphocytes T dépendants de l'IL-2, CA1
• Nom complet: protein tyrosine phosphatase, receptor type, C
• Numéro d’acquisition GenBank: BC014239
• Symbole du gène: CD45
• Identification du gène (NCBI): 5788
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RO is the shortest CD45 isoform (lacking all three exons) and is expressed by activated and memory T cells, thymocytes, some B cells, and weakly on granulocytes and macrophage (PMID: 27100180; 12414720).

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