Anticorps Recombinant de lapin anti-CD45RO

CD45RO Recombinant Antibody for FC

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

FC

Conjugaison

PerCP-Cyanine5.5 Fluorescent Dye

CloneNo.

UCHL1

N° de cat : CPY5-65613

Synonymes

CD45, EC:3.1.3.48, GP180, L CA, LCA

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Galerie de données de validation

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  FC experiment of human PBMCs using CPY5-65613

  1x10^6 human PBMCs were surface stained with FITC Plus Anti-Human CD45RA and 5 ul PerCP-Cyanine5.5 Anti-Human CD45RO (UCHL1) Mouse IgG2a Recombinant Antibody (CPY5-65613, Clone:UCHL1) or unstained. Cells were not fixed. Lymphocytes were gated.

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  FC experiment of human PBMCs using CPY5-65613

  1x10^6 human PBMCs were surface stained with 5 ul PerCP-Cyanine5.5 Anti-Human CD45RO (UCHL1) Mouse IgG2a Recombinant Antibody (CPY5-65613, Clone:UCHL1) (red) or unstained (blue). Cells were not fixed. Lymphocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CPY5-65613 cible CD45RO dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Lignée de lymphocytes T dépendants de l'IL-2, CA1
• Nom complet: protein tyrosine phosphatase, receptor type, C
• Numéro d’acquisition GenBank: BC014239
• Symbole du gène: CD45
• Identification du gène (NCBI): 5788
• Conjugaison: PerCP-Cyanine5.5 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 482 nm / 690nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RO is the shortest CD45 isoform (lacking all three exons) and is expressed by activated and memory T cells, thymocytes, some B cells, and weakly on granulocytes and macrophage (PMID: 27100180; 12414720).

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