Anticorps Polyclonal de lapin anti-CD46

CD46 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 28936-1-AP

Synonymes

TRA2.10, TLX, MIC10, Membrane cofactor protein, MCP

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Galerie de données de validation

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  WB analysis using 28936-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 28936-1-AP (CD46 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 28936-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 28936-1-AP (CD46 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 28936-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 28936-1-AP (CD46 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 28936-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28936-1-AP (CD46 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 28936-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28936-1-AP (CD46 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 28936-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CD46 antibody (28936-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules Jurkat
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

28936-1-AP cible CD46 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD46 Protéine recombinante Ag30376
• Nom complet: CD46 molecule, complement regulatory protein
• Masse moléculaire calculée: 384 aa, 43 kDa
• Poids moléculaire observé: 50-70 kDa
• Numéro d’acquisition GenBank: BC030594
• Symbole du gène: CD46
• Identification du gène (NCBI): 4179
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD46, also known as Membrane cofactor protein (MCP) or MIC10, is a ubiquitously expressed type type-I transmembrane glycoprotein that acts as a cofactor for complement factor I to mediate inactivation of C3b and C4b deposited on host cells. CD46 is a receptor for a large number of pathogens, including measles virus and adenovirus (PMID: 20941397). CD46 plays a role in linking innate and adaptive immune responses. It may be involved in the fusion of the spermatozoa with the oocyte during fertilization. Mutations of the CD46 gene have been associated with some diseases including hemolytic uremic syndrome (PMID: 26054645). CD46 is expressed on most cells as four isoforms that are derived via alternative splicing (PMID: 15324743).

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