Anticorps Polyclonal de lapin anti-CD46

CD46 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 28936-1-PBS

Synonymes

TRA2.10, TLX, MIC10, Membrane cofactor protein, MCP

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Galerie de données de validation

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  WB analysis using 28936-1-AP (same clone as 28936-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 28936-1-AP (CD46 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28936-1-PBS in a different storage buffer formulation.

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  WB analysis using 28936-1-AP (same clone as 28936-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 28936-1-AP (CD46 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28936-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 28936-1-AP (same clone as 28936-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 28936-1-AP (CD46 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28936-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 28936-1-AP (same clone as 28936-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28936-1-AP (CD46 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28936-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 28936-1-AP (same clone as 28936-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28936-1-AP (CD46 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28936-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 28936-1-AP (same clone as 28936-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CD46 antibody (28936-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 28936-1-PBS in a different storage buffer formulation.

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Informations sur le produit

28936-1-PBS cible CD46 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD46 Protéine recombinante Ag30376
• Nom complet: CD46 molecule, complement regulatory protein
• Masse moléculaire calculée: 384 aa, 43 kDa
• Poids moléculaire observé: 50-70 kDa
• Numéro d’acquisition GenBank: BC030594
• Symbole du gène: CD46
• Identification du gène (NCBI): 4179
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD46, also known as Membrane cofactor protein (MCP) or MIC10, is a ubiquitously expressed type type-I transmembrane glycoprotein that acts as a cofactor for complement factor I to mediate inactivation of C3b and C4b deposited on host cells. CD46 is a receptor for a large number of pathogens, including measles virus and adenovirus (PMID: 20941397). CD46 plays a role in linking innate and adaptive immune responses. It may be involved in the fusion of the spermatozoa with the oocyte during fertilization. Mutations of the CD46 gene have been associated with some diseases including hemolytic uremic syndrome (PMID: 26054645). CD46 is expressed on most cells as four isoforms that are derived via alternative splicing (PMID: 15324743).

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