Anticorps Polyclonal de lapin anti-CD47

CD47 Polyclonal Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(36)

N° de cat : 20305-1-AP

Synonymes

MER6, Leukocyte surface antigen CD47, Integrin-associated protein, Integrin associated protein, IAP

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Galerie de données de validation

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  IHC staining of human prostate cancer using 20305-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20305-1-AP (CD47 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 20305-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20305-1-AP (CD47 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 20305-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20305-1-AP (CD47 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 20305-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20305-1-AP (CD47 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 26 publications below
• IHC: See 10 publications below
• IF: See 7 publications below

Informations sur le produit

20305-1-AP cible CD47 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD47 Protéine recombinante Ag14132
• Nom complet: CD47 molecule
• Masse moléculaire calculée: 323 aa, 35 kDa
• Numéro d’acquisition GenBank: BC010016
• Symbole du gène: CD47
• Identification du gène (NCBI): 961
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD47, also known as integrin-associated protein (IAP), is a member of the immunoglobulin superfamily containing a five-pass transmembrane attachment. CD47 is heavily glycosylated and widely expressed by hematopoietic and nonhematopoietic cells. CD47 interacts with several membrane integrins and also acts as a receptor for thrombospondin (THBS1). It is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. CD47 also functions as a ligand for signal regulatory protein-α (SIRPα). Upon binding CD47, SIRPα initiates a signaling cascade that results in the inhibition of phagocytosis.

Publications

Species	Application	Title
mouse	WB	Theranostics; Bioengineering CXCR4-overexpressing cell membrane functionalized ROS-responsive nanotherapeutics for targeting cerebral ischemia-reperfusion injury.; Authors - Li Luo
human	WB	ACS Appl Mater Interfaces; PMID: 31718159; Authors - Tengfei Wang
human	WB	J Control Release; Engineered biomimetic nanoreactor for synergistic photodynamic-chemotherapy against hypoxic tumor; Authors - Haoyu Guo
mouse	WB	Biomater Sci; 4T1 cell membrane fragment reunited PAMAM polymer units disguised as tumor cell clusters for tumor homotypic targeting and anti-metastasis treatment; Authors - Xiaochen Pei
human	IHC	Commun Biol; RAGA prevents tumor immune evasion of LUAD by promoting CD47 lysosome degradation; Authors - Lian Zhang
human	IF	Front Oncol; Identification of Schlafen-11 as a Target of CD47 Signaling That Regulates Sensitivity to Ionizing Radiation and Topoisomerase Inhibitors.; Authors - Sukhbir Kaur

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