Anticorps Monoclonal anti-CD47

CD47 Monoclonal Antibody for IF/ICC, FC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

IF/ICC, FC, ELISA

Conjugaison

Non conjugué

CloneNo.

1A4C4

N° de cat : 60551-1-Ig

Synonymes

1A4C4, CD47 molecule, IAP, Integrin associated protein, Integrin-associated protein

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Galerie de données de validation

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  IF Staining of A431 using 60551-1-Ig

  Immunofluorescent analysis of un-fixed A431 cells using CD47 antibody (60551-1-Ig, Clone: 1A4C4 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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  IF Staining of A431 using 60551-1-Ig

  Immunofluorescent analysis of un-fixed A431 cells using CD47 antibody (60551-1-Ig, Clone: 1A4C4 ) at dilution of 1:500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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  IF Staining of Jurkat using 60551-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD47 antibody (60551-1-Ig, Clone: 1A4C4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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  FC experiment of human PBMCs using 60551-1-Ig

  1x10^6 human PBMCs were surface stained with 0.2 μg CD47 Monoclonal Antibody (60551-1-Ig, Clone: 1A4C4, right) and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, left) was parallel stained as control. Cells were not fixed.

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Applications testées

• Résultats positifs en IF/ICC: cellules HepG2, cellules A431, cellules Jurkat
• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

60551-1-Ig cible CD47 dans les applications de IF/ICC, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD47 Protéine recombinante Eg31499
• Nom complet: CD47 molecule
• Masse moléculaire calculée: 323 aa, 35 kDa
• Numéro d’acquisition GenBank: BC010016
• Symbole du gène: CD47
• Identification du gène (NCBI): 961
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD47, also known as integrin-associated protein (IAP), is a member of the immunoglobulin superfamily containing a five-pass transmembrane attachment. CD47 is heavily glycosylated and widely expressed by hematopoietic and nonhematopoietic cells. CD47 interacts with several membrane integrins and also acts a receptor for thrombospondin (THBS1). It is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. CD47 also functions as a ligand for signal regulatory protein-α (SIRPα). Upon binding CD47, SIRPα initiates a signaling cascade that results in the inhibition of phagocytosis.

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