Anticorps Recombinant de lapin anti-CD47

CD47 Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242850C4

N° de cat : 85958-1-PBS

Synonymes

IAP, Integrin-associated protein, Leukocyte surface antigen CD47

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Galerie de données de validation

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  WB analysis of mouse spleen using 85958-1-RR (same clone as 85958-1-PBS)

  Mouse spleen tissue was subjected to SDS PAGE followed by western blot with 85958-1-RR (CD47 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85958-1-PBS in a different storage buffer formulation.

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  IF Staining of RAW 264.7 using 85958-1-RR (same clone as 85958-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CD47 antibody (85958-1-RR, Clone: 242850C4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85958-1-PBS in a different storage buffer formulation.

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Informations sur le produit

85958-1-PBS cible CD47 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD47 Protéine recombinante Eg1394
• Nom complet: CD47 antigen (Rh-related antigen, integrin-associated signal transducer)
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 45-55 kDa
• Numéro d’acquisition GenBank: NP_001355344.1
• Symbole du gène: Cd47
• Identification du gène (NCBI): 16423
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD47, also known as integrin-associated protein (IAP), is a member of the immunoglobulin superfamily containing a five-pass transmembrane attachment. CD47 is heavily glycosylated and widely expressed by hematopoietic and nonhematopoietic cells. CD47 interacts with several membrane integrins and also acts as a receptor for thrombospondin (THBS1). It is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. CD47 also functions as a ligand for signal regulatory protein-α (SIRPα). Upon binding CD47, SIRPα initiates a signaling cascade that results in the inhibition of phagocytosis.

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