Anticorps Recombinant de lapin anti-CD47

CD47 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC

Conjugaison

Non conjugué

CloneNo.

242850C4

N° de cat : 98456-3-RR

Synonymes

IAP, Integrin-associated protein, Leukocyte surface antigen CD47

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Galerie de données de validation

• 

  FC experiment of mouse splenocytes using 98456-3-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD47 Rabbit RecAb (98456-3-RR, Clone:242850C4) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using 98456-3-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD47 Rabbit RecAb (98456-3-RR, Clone:242850C4) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98456-3-RR

  Biolayer interferometry (BLl) kinetic assays of 98456-3-RR against Mouse CD47 were performed. The affinity constant is 0.273 nM.

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Applications testées

• Résultats positifs en cytométrie: splénocytes de souris,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98456-3-RR cible CD47 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD47 Protéine recombinante Eg1394
• Nom complet: CD47 antigen (Rh-related antigen, integrin-associated signal transducer)
• Masse moléculaire calculée: 33 kDa
• Numéro d’acquisition GenBank: NP_001355344.1
• Symbole du gène: Cd47
• Identification du gène (NCBI): 16423
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

CD47, also known as integrin-associated protein (IAP), is a member of the immunoglobulin superfamily containing a five-pass transmembrane attachment. CD47 is heavily glycosylated and widely expressed by hematopoietic and nonhematopoietic cells. CD47 interacts with several membrane integrins and also acts as a receptor for thrombospondin (THBS1). It is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. CD47 also functions as a ligand for signal regulatory protein-α (SIRPα). Upon binding CD47, SIRPα initiates a signaling cascade that results in the inhibition of phagocytosis.

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