Anticorps Recombinant de lapin anti-CD47

CD47 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

CoraLite® Plus 405 Fluorescent Dye

CloneNo.

240997D8

N° de cat : CL405-98131

Synonymes

240997D8, CD47 molecule, IAP, Integrin associated protein, Integrin-associated protein

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Galerie de données de validation

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  FC experiment of human PBMCs using CL405-98131

  1x10^6 FC receptor blocked human PBMCs were surface stained with 5 ul CoraLite® Plus 405 Anti-Human CD47 Rabbit RecAb (CL405-98131, Clone: 240997D8) or CoraLite® Plus 405 Rabbit IgG Isotype Control RecAb (CL405-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using CL405-98131

  1x10^6 FC receptor blocked human PBMCs were surface stained with 5 ul CoraLite® Plus 405 Anti-Human CD47 Rabbit RecAb (CL405-98131, Clone: 240997D8) (red) or CoraLite® Plus 405 Rabbit IgG Isotype Control RecAb (CL405-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL405-98131 cible CD47 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD47 Protéine recombinante Eg31499
• Nom complet: CD47 molecule
• Masse moléculaire calculée: 323 aa, 35 kDa
• Numéro d’acquisition GenBank: BC010016
• Symbole du gène: CD47
• Identification du gène (NCBI): 961
• Conjugaison: CoraLite® Plus 405 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 399 nm / 422 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD47, also known as integrin-associated protein (IAP), is a member of the immunoglobulin superfamily containing a five-pass transmembrane attachment. CD47 is heavily glycosylated and widely expressed by hematopoietic and nonhematopoietic cells. CD47 interacts with several membrane integrins and also acts as a receptor for thrombospondin (THBS1). It is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. CD47 also functions as a ligand for signal regulatory protein-α (SIRPα). Upon binding CD47, SIRPα initiates a signaling cascade that results in the inhibition of phagocytosis.

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