Anticorps Polyclonal de lapin anti-CD48

CD48 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 31774-1-PBS

Synonymes

BLAST, BLAST 1, BLAST1, CD48 antigen, MEM 102

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Galerie de données de validation

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  WB analysis using 31774-1-AP (same clone as 31774-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31774-1-AP (CD48/SLAMF2 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31774-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 31774-1-AP (same clone as 31774-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31774-1-AP (CD48 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31774-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 31774-1-AP (same clone as 31774-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31774-1-AP (CD48 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31774-1-PBS in a different storage buffer formulation.

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Informations sur le produit

31774-1-PBS cible CD48 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD48 Protéine recombinante Eg1323
• Nom complet: CD48 antigen
• Masse moléculaire calculée: 27 kDa
• Poids moléculaire observé: 45-55 kDa
• Numéro d’acquisition GenBank: BAE96326.1
• Symbole du gène: Cd48
• Identification du gène (NCBI): 12506
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Signaling lymphocytic activation molecule family 2 (SLAMF2, CD48) is an adhesion and costimulatory molecule expressed constitutively on most hematopoietic cells, particularly in antigen-presenting cells (APC). CD48 is expressed on all the hematopoietic cells, both in humans and mice, except for murine neutrophils and long-term HSC. CD48 exists as both a membrane-bound (mCD48) and a soluble (sCD48) form. CD48 can activate T cells, antigen-presenting cells and granulocytes, by binding to CD2 or bacterial FimH, and through cell intrinsic effects. Interactions between CD48 and its high-affinity ligand CD244 are more complex, with both stimulatory and inhibitory outcomes. CD48 expression is increased in autoimmunity and allergy diseases, and anti-CD48 monoclonal antibodies have been shown to attenuate experimental autoimmune encephalomyelitis.

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