Anticorps Polyclonal de lapin anti-CD48

CD48 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Avis (2)

N° de cat : 31945-1-AP

Synonymes

BLAST, BLAST1, B-lymphocyte activation marker BLAST-1, CD48 antigen, CD48 molecule

---

Galerie de données de validation

• 

  WB analysis using 31945-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31945-1-AP (CD48 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 31945-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 31945-1-AP (CD48 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 31945-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 31945-1-AP (CD48 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of Jurkat using 31945-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD48 antibody (31945-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

---

Applications testées

• Résultats positifs en WB: cellules Daudi, cellules Jurkat, cellules Raji, cellules Ramos
• Résultats positifs en IHC: tissu d'amygdalite humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

31945-1-AP cible CD48 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD48 Protéine recombinante Eg1005
• Nom complet: CD48 molecule
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 45-55 kDa
• Numéro d’acquisition GenBank: BC016182
• Symbole du gène: CD48
• Identification du gène (NCBI): 962
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Signaling lymphocytic activation molecule family 2 (SLAMF2, CD48) is an adhesion and costimulatory molecule expressed constitutively on most hematopoietic cells, particularly in antigen-presenting cells (APC). CD48 is expressed on all the hematopoietic cells, both in humans and mice, except for murine neutrophils and long-term HSC. CD48 exists as both a membrane-bound (mCD48) and a soluble (sCD48) form. CD48 can activate T cells, antigen-presenting cells and granulocytes, by binding to CD2 or bacterial FimH, and through cell intrinsic effects. Interactions between CD48 and its high-affinity ligand CD244 are more complex, with both stimulatory and inhibitory outcomes. CD48 expression is increased in autoimmunity and allergy diseases, and anti-CD48 monoclonal antibodies have been shown to attenuate experimental autoimmune encephalomyelitis.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Megan (Verified Customer) (07-24-2025)

Great for IF applications

• Applications: Immunohistochemistry, Immunofluorescence

FH

Alyona (Verified Customer) (07-23-2025)

The advisor was really helpful

• Applications: Immunohistochemistry, Immunofluorescence

{{ptg:RelatedPrimaryAntibodies}}