Anticorps Recombinant de lapin anti-CD48

CD48 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

241389F9

N° de cat : 84186-5-RR

Synonymes

CD48 molecule, CD48 antigen, B-lymphocyte activation marker BLAST-1, BLAST1, BLAST

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Galerie de données de validation

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  WB analysis using 84186-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84186-5-RR (CD48 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84186-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84186-5-RR against Human CD48 were performed. The affinity constant is 0.672 nM.

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Applications testées

• Résultats positifs en WB: cellules Ramos, cellules Daudi, cellules Jurkat, cellules Raji

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84186-5-RR cible CD48 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: CD48 molecule
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 40-45 kDa
• Numéro d’acquisition GenBank: BC016182
• Symbole du gène: CD48
• Identification du gène (NCBI): 962
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Signaling lymphocytic activation molecule family 2 (SLAMF2, CD48) is an adhesion and costimulatory molecule expressed constitutively on most hematopoietic cells, particularly in antigen-presenting cells (APC). CD48 is expressed on all the hematopoietic cells, both in humans and mice, except for murine neutrophils and long-term HSC. CD48 exists as both a membrane-bound (mCD48) and a soluble (sCD48) form. CD48 can activate T cells, antigen-presenting cells and granulocytes, by binding to CD2 or bacterial FimH, and through cell intrinsic effects. Interactions between CD48 and its high-affinity ligand CD244 are more complex, with both stimulatory and inhibitory outcomes. CD48 expression is increased in autoimmunity and allergy diseases, and anti-CD48 monoclonal antibodies have been shown to attenuate experimental autoimmune encephalomyelitis.

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