Anticorps Recombinant de lapin anti-CD48

CD48 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC

Conjugaison

Non conjugué

CloneNo.

240732A5

N° de cat : 98081-1-RR

Synonymes

MEM 102, CD48 antigen, BLAST1, BLAST 1, BLAST

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Galerie de données de validation

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  FC experiment of mouse splenocytes using 98081-1-RR

  1x10^6 mouse splenocytes were surface stained with 0.15 ug Anti-Mouse CD48 Rabbit Recombinant Antibody (98081-1-RR, Clone: 240732A5) or Isotype Control, and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD19. Cells were not fixed.

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  FC experiment of mouse splenocytes using 98081-1-RR

  1x10^6 mouse splenocytes were surface stained with 0.15 ug Anti-Mouse CD48 Rabbit Recombinant Antibody (98081-1-RR, Clone:240732A5) (red) or Isotype Control (blue), and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98081-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98081-1-RR against Mouse CD48 were performed. The affinity constant is 1.48 nM.

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Applications testées

• Résultats positifs en cytométrie: splénocytes de souris,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98081-1-RR cible CD48 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: CD48 antigen
• Masse moléculaire calculée: 27 kDa
• Numéro d’acquisition GenBank: BAE96326.1
• Symbole du gène: Cd48
• Identification du gène (NCBI): 12506
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

Signaling lymphocytic activation molecule family 2 (SLAMF2, CD48) is an adhesion and costimulatory molecule expressed constitutively on most hematopoietic cells, particularly in antigen-presenting cells (APC). CD48 is expressed on all the hematopoietic cells, both in humans and mice, except for murine neutrophils and long-term HSC. CD48 exists as both a membrane-bound (mCD48) and a soluble (sCD48) form. CD48 can activate T cells, antigen-presenting cells and granulocytes, by binding to CD2 or bacterial FimH, and through cell intrinsic effects. Interactions between CD48 and its high-affinity ligand CD244 are more complex, with both stimulatory and inhibitory outcomes. CD48 expression is increased in autoimmunity and allergy diseases, and anti-CD48 monoclonal antibodies have been shown to attenuate experimental autoimmune encephalomyelitis.

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