Anticorps Recombinant de lapin anti-CD48

CD48 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

240732E3

N° de cat : 83871-1-RR

Synonymes

MEM 102, CD48 antigen, BLAST1, BLAST 1, BLAST

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Galerie de données de validation

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  WB analysis using 83871-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83871-1-RR (CD48 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of EL-4-B5 using 83871-1-RR

  Immunofluorescent analysis of (4% PFA) fixed EL-4-B5 cells using CD48 antibody (83871-1-RR, Clone: 240732E3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 83871-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83871-1-RR against Mouse CD48 were performed. The affinity constant is 0.629 nM.

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Applications testées

• Résultats positifs en WB: CTLL-2 cells, tissu splénique de souris
• Résultats positifs en IF/ICC: EL-4-B5 cells,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83871-1-RR cible CD48 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: CD48 antigen
• Masse moléculaire calculée: 27 kDa
• Poids moléculaire observé: 45-60 kDa
• Numéro d’acquisition GenBank: BAE96326.1
• Symbole du gène: Cd48
• Identification du gène (NCBI): 12506
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Signaling lymphocytic activation molecule family 2 (SLAMF2, CD48) is an adhesion and costimulatory molecule expressed constitutively on most hematopoietic cells, particularly in antigen-presenting cells (APC). CD48 is expressed on all the hematopoietic cells, both in humans and mice, except for murine neutrophils and long-term HSC. CD48 exists as both a membrane-bound (mCD48) and a soluble (sCD48) form. CD48 can activate T cells, antigen-presenting cells and granulocytes, by binding to CD2 or bacterial FimH, and through cell intrinsic effects. Interactions between CD48 and its high-affinity ligand CD244 are more complex, with both stimulatory and inhibitory outcomes. CD48 expression is increased in autoimmunity and allergy diseases, and anti-CD48 monoclonal antibodies have been shown to attenuate experimental autoimmune encephalomyelitis.

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