Anticorps Monoclonal anti-CD49b

CD49b Monoclonal Antibody for FC, ELISA

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain

Applications

FC, ELISA

Conjugaison

Non conjugué

CloneNo.

AK7

N° de cat : 65252-1-Ig

Synonymes

BR, CD49B, Collagen receptor, GPIa, Integrin alpha 2, ITGA2, VLA 2, VLA 2 subunit alpha, VLAA2

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Galerie de données de validation

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  FC experiment of human PBMCs using 65252-1-Ig

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD49b (65252-1-Ig, Clone:AK7) and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:500. Cells were not fixed. Monocytes were gated.

• 

  FC experiment of human PBMCs using 65252-1-Ig

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD49b (65252-1-Ig, Clone:AK7) and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:500. Cells were not fixed. Monocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

65252-1-Ig cible CD49b dans les applications de FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Plaquettes humaines
• Nom complet: integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor)
• Masse moléculaire calculée: 126 kDa
• Numéro d’acquisition GenBank: NM_002203
• Symbole du gène: Integrin alpha 2
• Identification du gène (NCBI): 3673
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

CD49b, also known as alpha2 integrin, is a type I transmembrane glycoprotein which forms an adhesion molecule VLA-2 with beta1 integrin. CD49b is a receptor for many (extracellular) matrix and non-matrix molecules (PMID: 23847623). CD49b is expressed on numerous cell types including platelets, activated T cells, B cells, and monocytes. CD49b activation skews NK cell effector function to a phenotype that exhibits elevated IFN-γ production with a concomitant inhibition of motility and cytolytic function (PMID: 32971468, 32762681). The Anti-Human CD49b antibody, clone AK7, is derived from Human platelets.

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