Anticorps Monoclonal anti-CD49b

CD49b Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgM, kappa

Réactivité testée

souris

Applications

FC

Conjugaison

APC Fluorescent Dye

Publications

1

CloneNo.

DX5

N° de cat : APC-65065

Synonymes

CD49B, DX5, integrin alpha 2, Itga2

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Galerie de données de validation

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  FC experiment of mouse splenocytes using APC-65065

  1X10^6 C57BL/6 mouse splenocytes were surface stained with FITC Anti-Mouse NK1.1 (CD161) (FITC-65138, Clone: PK136) and 0.20 ug APC Anti-Mouse CD49b (APC-65065, Clone: DX5). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using APC-65065

  1X10^6 C57BL/6 mouse splenocytes were surface stained with FITC Anti-Mouse NK1.1 (CD161) (FITC-65138, Clone: PK136) and 0.20 ug APC-Rat IgM isotype control antibody. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: splénocytes de souris,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• FC: See 1 publications below

Informations sur le produit

APC-65065 cible CD49b dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Rat / IgM, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cellules NK isolées de souris C57BL/6
• Nom complet: integrin alpha 2
• Numéro d’acquisition GenBank: BC065139
• Symbole du gène: Itga2
• Identification du gène (NCBI): 16398
• Conjugaison: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS avec azoture de sodium à 0,1 % et un agent stabilisant.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Integrins are a large family of α/β transmembrane receptors playing a key role in cell-cell interactions and cell adhesion to the extracellular matrix (PMID: 30374344). CD49b, also known as integrin alpha-2, is a 150 kDa transmembrane glycoprotein expressed on a wide variety of cell types including platelets, megakaryocytes, NK cells, NKT cells, neutrophils, and long-term activated T cells (PMID: 18802064; 2415516). Integrin alpha-2/beta-1 (also known as VLA-2) is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin (PMID: 2557634). It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and CLG gene expression, force generation and organization of newly synthesized extracellular matrix.

Publications

Species	Application	Title
	FC	Liver Int; Human poliovirus receptor contributes to biliary atresia pathogenesis by exacerbating natural-killer-cell-mediated bile duct injury; Authors - Yuan Li