Anticorps Monoclonal anti-CD49b

CD49b Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain

Applications

FC

Conjugaison

PE Fluorescent Dye

CloneNo.

AK7

N° de cat : PE-65252

Synonymes

BR, CD49B, Collagen receptor, GPIa, Integrin alpha 2, ITGA2, VLA 2, VLA 2 subunit alpha, VLAA2

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Galerie de données de validation

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  FC experiment of human PBMCs using PE-65252

  1X10^6 human PBMCs were surface stained with 5 ul PE Anti-Human CD49b (PE-65252, Clone:AK7) or unstained. Cells were not fixed. Monocytes were gated.

• 

  FC experiment of human PBMCs using PE-65252

  1X10^6 human PBMCs were surface stained with 5 ul PE Anti-Human CD49b (PE-65252, Clone:AK7) or unstained. Cells were not fixed. Monocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

PE-65252 cible CD49b dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Plaquettes humaines
• Nom complet: integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor)
• Masse moléculaire calculée: 126 kDa
• Numéro d’acquisition GenBank: NM_002203
• Symbole du gène: Integrin alpha 2
• Identification du gène (NCBI): 3673
• Conjugaison: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Forme: Liquide
• Méthode de purification: N/A
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD49b, also known as alpha2 integrin, is a type I transmembrane glycoprotein which forms an adhesion molecule VLA-2 with beta1 integrin. CD49b is a receptor for many (extracellular) matrix and non-matrix molecules (PMID: 23847623). CD49b is expressed on numerous cell types including platelets, activated T cells, B cells, and monocytes. CD49b activation skews NK cell effector function to a phenotype that exhibits elevated IFN-γ production with a concomitant inhibition of motility and cytolytic function (PMID: 32971468, 32762681). The Anti-Human CD49b antibody, clone AK7, is derived from Human platelets.

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