Anticorps Recombinant de lapin anti-CD5

CD5 Recombinant Antibody for FC

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

L17F12

N° de cat : 65530-1-PBS

Synonymes

CD5 molecule, L17F12, LEU1, Lymphocyte antigen T1/Leu 1, Lymphocyte antigen T1/Leu-1

---

Galerie de données de validation

• 

  FC experiment of human PBMCs using 65530-1-MR (same clone as 65530-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD5 Mouse Recombinant Antibody (65530-1-MR, Clone:L17F12) or Mouse IgG1 Isotype Control, and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CL488-conjugated Anti-Human CD19. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65530-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65530-1-PBS

  1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD5 Mouse Recombinant Antibody (65530-1-PBS, Clone:L17F12) or Mouse IgG1 Isotype Control, and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CL488-conjugated Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65530-1-PBS

  1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD5 Mouse Recombinant Antibody (65530-1-PBS, Clone:L17F12) or Mouse IgG1 Isotype Control, and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65530-1-MR (same clone as 65530-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD5 Mouse Recombinant Antibody (65530-1-MR, Clone:L17F12) or Mouse IgG1 Isotype Control, and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65530-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65530-1-MR (same clone as 65530-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD5 Mouse Recombinant Antibody (65530-1-MR, Clone:L17F12) (left) or with 0.1 ug Anti-Human CD5 Mouse Monoclonal Recombinant Antibody (Clone:L17F12) (center and right) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CL488-conjugated Anti-Human CD19. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65530-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65530-1-PBS

  1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD5 Mouse Recombinant Antibody (65530-1-PBS, Clone:L17F12) (left) or with 0.1 ug Anti-Human CD5 Mouse Monoclonal Recombinant Antibody (Clone:L17F12) (center and right) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CL488-conjugated Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.

---

Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

65530-1-PBS cible CD5 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Cellules humaines de leucémie lymphoblastique aiguë à lymphocytes T (LAL-T)
• Nom complet: CD5 molecule
• Masse moléculaire calculée: 495 aa, 55 kDa
• Numéro d’acquisition GenBank: BC027901
• Symbole du gène: CD5
• Identification du gène (NCBI): 921
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. Stable for one year after shipment.

Informations générales

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).

{{ptg:RelatedPrimaryAntibodies}}