Anticorps Recombinant de lapin anti-CD5

CD5 Recombinant Antibody for FC

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

L17F12

N° de cat : 65599-1-PBS

Synonymes

CD5 molecule, L17F12, LEU1, Lymphocyte antigen T1/Leu 1, Lymphocyte antigen T1/Leu-1

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Galerie de données de validation

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  FC experiment of human PBMCs using 65599-1-MR (same clone as 65599-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD5 Mouse IgG2a Recombinant Antibody (65599-1-MR, Clone: L17F12) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65599-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65599-1-MR (same clone as 65599-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD5 Mouse IgG2a Recombinant Antibody (65599-1-MR, Clone:L17F12) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65599-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65599-1-MR (same clone as 65599-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD5 Mouse IgG2a Recombinant Antibody (65599-1-MR, Clone:L17F12) (left) or 0.25 ug Anti-Human CD5 (65200-1-Ig, Clone: L17F12) (right) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65599-1-PBS in a different storage buffer formulation.

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Informations sur le produit

65599-1-PBS cible CD5 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Cellules humaines de leucémie lymphoblastique aiguë à lymphocytes T (LAL-T)
• Nom complet: CD5 molecule
• Masse moléculaire calculée: 495 aa, 55 kDa
• Numéro d’acquisition GenBank: BC027901
• Symbole du gène: CD5
• Identification du gène (NCBI): 921
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C.

Informations générales

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).

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