Anticorps Recombinant de lapin anti-CD5

CD5 Recombinant Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

240244C2

N° de cat : 83451-4-PBS

Synonymes

Lyt 1, Ly A, Ly 12, Ly 1, CD5 antigen

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Galerie de données de validation

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  WB analysis of EL-4 using 83451-4-RR (same clone as 83451-4-PBS)

  EL-4 cells were subjected to SDS PAGE followed by western blot with 83451-4-RR (CD5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83451-4-PBS in a different storage buffer formulation.

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  WB analysis of mouse thymus using 83451-4-RR (same clone as 83451-4-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 83451-4-RR (CD5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83451-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 83451-4-RR (same clone as 83451-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83451-4-RR (CD5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83451-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 83451-4-RR (same clone as 83451-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83451-4-RR (CD5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83451-4-PBS in a different storage buffer formulation.

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  IF Staining of mouse spleen using 83451-4-RR (same clone as 83451-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD5 antibody (83451-4-RR, Clone: 240244C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83451-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83451-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83451-4-RR against Mouse CD5 were performed. The affinity constant is 89.9 pM.

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Informations sur le produit

83451-4-PBS cible CD5 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: CD5 antigen
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 55 kDa, 67 kDa
• Numéro d’acquisition GenBank: NM_007650.3
• Symbole du gène: Cd5
• Identification du gène (NCBI): 12507
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).

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