Anticorps Recombinant de lapin anti-CD5

CD5 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

240428G2

N° de cat : 83543-7-PBS

Synonymes

T1, Lymphocyte antigen T1/Leu-1, Lymphocyte antigen T1/Leu 1, LEU1, CD5 molecule

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Galerie de données de validation

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  WB analysis of Jurkat using 83543-7-RR (same clone as 83543-7-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 83543-7-RR (CD5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83543-7-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 83543-7-RR (same clone as 83543-7-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83543-7-RR (CD5 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83543-7-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 83543-7-RR (same clone as 83543-7-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83543-7-RR (CD5 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83543-7-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary cancer using 83543-7-RR (same clone as 83543-7-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83543-7-RR (CD5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83543-7-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary cancer using 83543-7-RR (same clone as 83543-7-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83543-7-RR (CD5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83543-7-PBS in a different storage buffer formulation.

• 

  IF Staining of Jurkat using 83543-7-RR (same clone as 83543-7-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD5 antibody (83543-7-RR, Clone: 240428G2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83543-7-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83543-7-RR

  Biolayer interferometry (BLl) kinetic assays of 83543-7-RR against Human CD5 were performed. The affinity constant is 2.09 nM.

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Informations sur le produit

83543-7-PBS cible CD5 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: CD5 molecule
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: NM_014207.4
• Symbole du gène: CD5
• Identification du gène (NCBI): 921
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).

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