Anticorps Recombinant de lapin anti-CD5

CD5 Recombinant Antibody for FC

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

FC

Conjugaison

FITC Plus Fluorescent Dye

CloneNo.

L17F12

N° de cat : FITC-65530

Synonymes

CD5 molecule, L17F12, LEU1, Lymphocyte antigen T1/Leu 1, Lymphocyte antigen T1/Leu-1

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Galerie de données de validation

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  FC experiment of human PBMCs using FITC-65530

  1x10^6 human PBMCs were surface stained with CL750 Anti-Human CD19 and 5 ul FITC Plus Anti-Human CD5 Mouse Recombinant Antibody (FITC-65530, Clone:L17F12) or FITC Plus Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

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  FC experiment of human PBMCs using FITC-65530

  1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD5 Mouse Recombinant Antibody (FITC-65530, Clone:L17F12) or FITC Plus Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using FITC-65530

  1x10^6 human PBMCs were surface stained with CL750 Anti-Human CD19 and 5 ul FITC Plus Anti-Human CD5 Mouse Recombinant Antibody (FITC-65530, Clone:L17F12) (left) or 5 ul FITC Plus Anti-Human CD5 Mouse Monoclonal Antibody (FITC-65200, Clone:L17F12) (right). Cells were not fixed. Lymphocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

FITC-65530 cible CD5 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Cellules humaines de leucémie lymphoblastique aiguë à lymphocytes T (LAL-T)
• Nom complet: CD5 molecule
• Masse moléculaire calculée: 495 aa, 55 kDa
• Numéro d’acquisition GenBank: BC027901
• Symbole du gène: CD5
• Identification du gène (NCBI): 921
• Conjugaison: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).

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