Anticorps Recombinant de lapin anti-CD53

CD53 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250105D10

N° de cat : 85838-3-PBS

Synonymes

Cell surface glycoprotein CD53, Leukocyte surface antigen CD53

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Galerie de données de validation

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  WB analysis of RAW 264.7 using 85838-3-RR (same clone as 85838-3-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 85838-3-RR (CD53 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85838-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 85838-3-RR (same clone as 85838-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85838-3-RR (Cd53 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85838-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 85838-3-RR (same clone as 85838-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85838-3-RR (Cd53 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85838-3-PBS in a different storage buffer formulation.

• 

  IF Staining of A20 using 85838-3-RR (same clone as 85838-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A20 cells using Cd53 antibody (85838-3-RR, Clone: 250105D10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85838-3-PBS in a different storage buffer formulation.

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Informations sur le produit

85838-3-PBS cible CD53 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD53 Protéine recombinante Eg2684
• Nom complet: CD53 antigen
• Masse moléculaire calculée: 24 kDa
• Poids moléculaire observé: 35-40 kDa
• Numéro d’acquisition GenBank: NM_007651.3
• Symbole du gène: Cd53
• Identification du gène (NCBI): 12508
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD53 is also named as MOX44 and Tetraspanin-25 (Tspan-25). Tetraspanin CD53 is a member of the tetraspanin superfamily expressed exclusively within the immune compartment (PMID: 32440787). T cells depend on the phosphatase CD45 to initiate T cell receptor signaling. CD53 is shown to stabilize CD45 on the membrane and is required for optimal phosphatase activity and subsequent Lck activation. Together, CD53 as a regulator of CD45 activity required for T cell immunity (PMID: 35767951). CD53 is a marker for positively selected CD4+ CD8+ thymocytes (PMID: 11867561).

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