Anticorps Recombinant de lapin anti-CD53

CD53 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC

Conjugaison

Non conjugué

CloneNo.

250105D10

N° de cat : 98436-3-RR

Synonymes

Cell surface glycoprotein CD53, Leukocyte surface antigen CD53

---

Galerie de données de validation

• 

  FC experiment of mouse splenocytes using 98436-3-RR

  1x10^6 mouse splenocytes were surface stained with 0.125 ug Anti-Mouse Cd53 Rabbit RecAb (98436-3-RR, Clone:250105D10) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 488 Anti-Mouse CD4. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of mouse splenocytes using 98436-3-RR

  1x10^6 mouse splenocytes were surface stained with 0.125 ug Anti-Mouse Cd53 Rabbit RecAb (98436-3-RR, Clone: 250105D10) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9)(blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

---

Applications testées

• Résultats positifs en cytométrie: splénocytes de souris

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98436-3-RR cible CD53 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD53 Protéine recombinante Eg2684
• Nom complet: CD53 antigen
• Masse moléculaire calculée: 24 kDa
• Numéro d’acquisition GenBank: NM_007651.3
• Symbole du gène: Cd53
• Identification du gène (NCBI): 12508
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

CD53 is also named as MOX44 and Tetraspanin-25 (Tspan-25). Tetraspanin CD53 is a member of the tetraspanin superfamily expressed exclusively within the immune compartment (PMID: 32440787). T cells depend on the phosphatase CD45 to initiate T cell receptor signaling. CD53 is shown to stabilize CD45 on the membrane and is required for optimal phosphatase activity and subsequent Lck activation. Together, CD53 as a regulator of CD45 activity required for T cell immunity (PMID: 35767951). CD53 is a marker for positively selected CD4+ CD8+ thymocytes (PMID: 11867561).

{{ptg:RelatedPrimaryAntibodies}}