Anticorps Recombinant de lapin anti-ICAM-1/CD54

ICAM-1/CD54 Recombinant Antibody for FC

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

15.2

N° de cat : 65567-1-PBS

Synonymes

CD54, ICAM-1, 15.2, BB2, ICAM 1

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Galerie de données de validation

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  FC experiment of human PBMCs using 65567-1-MR (same clone as 65567-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human ICAM-1/CD54 (15.2) Mouse IgG2a RecAb (65567-1-MR, Clone:15.2) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4), and PE-conjugated Goat Anti-Mouse IgG. Cells were then stained with CoraLite® Plus 647 Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (CL647-65569, Clone: OKT3). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65567-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65567-1-MR (same clone as 65567-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human ICAM-1/CD54 (15.2) Mouse IgG2a RecAb (65567-1-MR, Clone:15.2) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue), and PE-conjugated Goat Anti-Mouse IgG. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65567-1-PBS in a different storage buffer formulation.

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Informations sur le produit

65567-1-PBS cible ICAM-1/CD54 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Cellules synoviales rhumatoïdes et monocytes humains
• Nom complet: intercellular adhesion molecule 1
• Masse moléculaire calculée: 90 kDa
• Numéro d’acquisition GenBank: BC015969
• Symbole du gène: ICAM-1
• Identification du gène (NCBI): 3383
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. Stable for one year after shipment.

Informations générales

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).

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