Anticorps Monoclonal anti-CD59

• Phare
• Validé par KD/KO

CD59 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

1E3A1

N° de cat : 68222-1-PBS

Synonymes

1E3A1, 20 kDa homologous restriction factor, CD 59, CD59 antigen, CD59 glycoprotein

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Galerie de données de validation

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  WB analysis using 68222-1-Ig (same clone as 68222-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68222-1-Ig (CD59 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68222-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 68222-1-Ig (same clone as 68222-1-PBS)

  WB result of CD59 antibody (68222-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD59 transfected HeLa cells. This data was developed using the same antibody clone with 68222-1-PBS in a different storage buffer formulation.

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  WB analysis using 68222-1-Ig (same clone as 68222-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68222-1-Ig (CD59 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68222-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 68222-1-Ig (same clone as 68222-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68222-1-Ig (CD59 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68222-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 68222-1-Ig (same clone as 68222-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68222-1-Ig (CD59 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68222-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 68222-1-Ig (same clone as 68222-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68222-1-Ig (CD59 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68222-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 68222-1-Ig (same clone as 68222-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68222-1-Ig (CD59 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68222-1-PBS in a different storage buffer formulation.

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  IHC staining of human urothelial carcinoma using 68222-1-Ig (same clone as 68222-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 68222-1-Ig (CD59 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68222-1-PBS in a different storage buffer formulation.

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  IHC staining of human urothelial carcinoma using 68222-1-Ig (same clone as 68222-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 68222-1-Ig (CD59 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68222-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 68222-1-Ig (same clone as 68222-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68222-1-Ig (CD59 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68222-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 68222-1-Ig (same clone as 68222-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68222-1-Ig (CD59 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68222-1-PBS in a different storage buffer formulation.

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  IF Staining of human placenta using 68222-1-Ig (same clone as 68222-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CD59 antibody (68222-1-Ig, Clone: 1E3A1 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68222-1-PBS in a different storage buffer formulation.

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  IF Staining of human placenta using 68222-1-Ig (same clone as 68222-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CD59 antibody (68222-1-Ig, Clone: 1E3A1 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68222-1-PBS in a different storage buffer formulation.

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  IF Staining of human placenta using 68222-1-Ig (same clone as 68222-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CD59 antibody (68222-1-Ig, Clone: 1E3A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68222-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human placenta using 68222-1-Ig (same clone as 68222-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CD59 antibody (68222-1-Ig, Clone: 1E3A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68222-1-PBS in a different storage buffer formulation.

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  IF Staining of BxPC-3 using 68222-1-Ig (same clone as 68222-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed BxPC-3 cells using CD59 antibody (68222-1-Ig, Clone: 1E3A1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68222-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68222-1-PBS cible CD59 dans les applications de WB, IHC, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD59 Protéine recombinante Ag32834
• Nom complet: CD59 molecule, complement regulatory protein
• Masse moléculaire calculée: 19 kDa
• Poids moléculaire observé: 18-25 kDa
• Numéro d’acquisition GenBank: BC001506
• Symbole du gène: CD59
• Identification du gène (NCBI): 966
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD59, also named as MIC11, MIN1, MIN2, MIN3, MSK21, MIRL, MACIF, HRF20 and 1F5 antigen, is a cell surface molecule glycoprotein with MW 18-25 kDa. It acts as a determinant of proximal-distal cell identity. CD59 acts by binding to the C8 and/or C9 complements of the assembling membrane attack complex (MAC), thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. It is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.

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