Anticorps Recombinant de lapin anti-CD59

• Phare
• Validé par KD/KO

CD59 Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241310G8

N° de cat : 84447-1-PBS

Synonymes

20 kDa homologous restriction factor, 241310G8, CD 59, CD59 antigen, CD59 glycoprotein

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Galerie de données de validation

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  WB analysis using 84447-1-RR (same clone as 84447-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84447-1-RR (CD59 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84447-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 84447-1-RR (same clone as 84447-1-PBS)

  WB result of CD59 antibody (84447-1-RR; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD59 transfected HeLa cells. This data was developed using the same antibody clone with 84447-1-PBS in a different storage buffer formulation.

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  IF Staining of BxPC-3 using 84447-1-RR (same clone as 84447-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed BxPC-3 cells using CD59 antibody (84447-1-RR, Clone: 241310G8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84447-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84447-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84447-1-RR against Human CD59 were performed. The affinity constant is 1.59 nM.

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Informations sur le produit

84447-1-PBS cible CD59 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: CD59 molecule, complement regulatory protein
• Masse moléculaire calculée: 19 kDa
• Poids moléculaire observé: 18-20 kDa
• Numéro d’acquisition GenBank: BC001506
• Symbole du gène: CD59
• Identification du gène (NCBI): 966
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD59, also named as MIC11, MIN1, MIN2, MIN3, MSK21, MIRL, MACIF, HRF20 and 1F5 antigen, is a cell surface molecule glycoprotein with MW 18-25 kDa. It acts as a determinant of proximal-distal cell identity. CD59 acts by binding to the C8 and/or C9 complements of the assembling membrane attack complex (MAC), thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. It is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.

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