Anticorps Recombinant de lapin anti-CD59

CD59 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

241311A4

N° de cat : 98099-1-RR

Synonymes

20 kDa homologous restriction factor, 241311A4, CD 59, CD59 antigen, CD59 glycoprotein

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Galerie de données de validation

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  FC experiment of human PBMCs using 98099-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD59 Rabbit Recombinant Antibody (98099-1-RR, Clone:241311A4) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG, Cells were then stained with APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using 98099-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD59 Rabbit Recombinant Antibody (98099-1-RR, Clone:241311A4) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using 98099-1-RR

  1x10^6 FC receptor blocked human PBMCs were surface stained with 0.25 ug Anti-Human CD59 Rabbit Recombinant Antibody (98099-1-RR, Clone:241311A4) and PE-conjugated Goat Anti-Rabbit IgG, and 0.25 ug APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3), and 0.25 ug Isotype Control. Cells were not fixed.

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  Affinity and Kinetic Characterization of 98099-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98099-1-RR against Human CD59 were performed. The affinity constant is 0.888 nM.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98099-1-RR cible CD59 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: CD59 molecule, complement regulatory protein
• Masse moléculaire calculée: 19 kDa
• Numéro d’acquisition GenBank: BC001506
• Symbole du gène: CD59
• Identification du gène (NCBI): 966
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

CD59, also named as MIC11, MIN1, MIN2, MIN3, MSK21, MIRL, MACIF, HRF20 and 1F5 antigen, is a cell surface molecule glycoprotein with MW 18-25 kDa. It acts as a determinant of proximal-distal cell identity. CD59 acts by binding to the C8 and/or C9 complements of the assembling membrane attack complex (MAC), thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. It is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.

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